Hotspot

rpl32-trnL-UAG

SSC · 115718-116643 · 926 bp

Back to Ehretia

Pi 0.0189

MarkerSeek score 58.40

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 926
variable_sites: 26
pi: 0.0189
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 58.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TTTTCCCCGAGTTTGACCAG	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.0	57.9	1675-1679 bp	1.0	81.3
rpl32-trnL-UAG_p2	GGAAGGGATATTGGACAGCG	TCCTAAGAGCAGCGTGTCTA	55.0	50.0	58.1	57.9	1063-1064 bp	1.0	81.0
rpl32-trnL-UAG_p3	TTTTCCCCGAGTTTGACCAG	AGCAGCGTGTCTACCAATTT	50.0	45.0	58.0	57.8	1668-1672 bp	1.0	80.8
rpl32-trnL-UAG_p4	TTTTCCCCGAGTTTGACCAG	CCTAAGAGCAGCGTGTCTAC	50.0	55.0	58.0	57.8	1674-1678 bp	1.0	80.7
rpl32-trnL-UAG_p5	TTTTCCCCGAGTTTGACCAG	CTAAGAGCAGCGTGTCTACC	50.0	55.0	58.0	57.8	1673-1677 bp	1.0	80.7