Hotspot

clpP-psbB

LSC · 74537-74982 · 446 bp

Back to Ehretia

Pi 0.00749

MarkerSeek score 60.80

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 446
variable_sites: 5
pi: 0.00749
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	CGCACTATACGTCAACCCAA	CGATACCAAGGCAAACCCAT	50.0	50.0	58.0	58.2	524-542 bp	1.0	81.5
clpP-psbB_p2	CGCACTATACGTCAACCCAA	ACACGATACCAAGGCAAACC	50.0	50.0	58.0	58.5	527-545 bp	1.0	79.6
clpP-psbB_p3	CGTCAACCCAAGATGCATCT	CGATACCAAGGCAAACCCAT	50.0	50.0	58.3	58.2	515-533 bp	1.0	79.5
clpP-psbB_p4	CGCACTATACGTCAACCCAA	TGAACACGATACCAAGGCAA	50.0	45.0	58.0	57.4	530-548 bp	1.0	79.0
clpP-psbB_p5	CGCACTATACGTCAACCCAA	GCAACCAGAGCTGTATGCAT	50.0	50.0	58.0	58.6	596-614 bp	1.0	78.1