Hotspot

petA-psbJ

LSC · 65478-66477 · 1000 bp

Back to Ehretia

Pi 0.0114

MarkerSeek score 52.60

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1000
variable_sites: 17
pi: 0.0114
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CCCGGATCCTGCTACTAAGA	GTCTCTAGCACGACCACTTG	55.0	55.0	58.0	58.0	1632-1662 bp	1.0	81.1
petA-psbJ_p2	CCCGGATCCTGCTACTAAGA	AACTGGCCGTTTTGATCCTT	55.0	45.0	58.0	58.0	2043-2081 bp	1.0	80.9
petA-psbJ_p3	CCCGGATCCTGCTACTAAGA	ACTGGCCGTTTTGATCCTTT	55.0	45.0	58.0	58.0	2042-2080 bp	1.0	80.9
petA-psbJ_p4	TCAGAGCTATCGCCCCAATA	AACTGGCCGTTTTGATCCTT	50.0	45.0	58.0	58.0	2133-2171 bp	1.0	80.9
petA-psbJ_p5	TCAGAGCTATCGCCCCAATA	ACTGGCCGTTTTGATCCTTT	50.0	45.0	58.0	58.0	2132-2170 bp	1.0	80.9