Hotspot

trnF-GAA-ndhJ

LSC · 49923-50597 · 675 bp

Back to Ehretia

Pi 0.012

MarkerSeek score 60.80

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 675
variable_sites: 12
pi: 0.012
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	TAGGATGGCCCCTACGTAAG	55.0	55.0	58.0	58.0	805-810 bp	1.0	82.0
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	TAGGATGGCCCCTACGTAAG	50.0	55.0	58.1	58.0	769-774 bp	1.0	81.7
trnF-GAA-ndhJ_p3	CTGGTAGAGCAGAGGACTGA	TAGGATGGCCCCTACGTAAG	55.0	55.0	57.9	58.0	793-798 bp	1.0	81.0
trnF-GAA-ndhJ_p4	GGGATAGCTCAGCTGGTAGA	ATCATCCACGCTTGAAACGT	55.0	45.0	58.0	58.2	847-852 bp	1.0	80.4
trnF-GAA-ndhJ_p5	GGGATAGCTCAGCTGGTAGA	GATAGGATGGCCCCTACGTA	55.0	55.0	58.0	57.8	807-812 bp	1.0	80.2