Hotspot

atpH-atpI

LSC · 14108-15231 · 1124 bp

Back to Ehretia

Pi 0.0102

MarkerSeek score 66.50

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1124
variable_sites: 17
pi: 0.0102
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	CGCGGCGTATATAGGTGAAT	45.0	50.0	58.0	57.7	1185-1204 bp	1.0	79.6
atpH-atpI_p2	TACCCTCTACAGCTTGACCC	CGCGGCGTATATAGGTGAAT	55.0	50.0	58.1	57.7	1268-1287 bp	1.0	78.7
atpH-atpI_p3	GCGATACCCTCTACAGCTTG	CGCGGCGTATATAGGTGAAT	55.0	50.0	57.9	57.7	1272-1291 bp	1.0	78.7
atpH-atpI_p4	AATAACGGAAGCGGCAGAAA	CGCGGCGTATATAGGTGAAT	45.0	50.0	58.2	57.7	1186-1205 bp	1.0	78.5
atpH-atpI_p5	GCCAATCCAGCAGCAATAAC	CGCGGCGTATATAGGTGAAT	50.0	50.0	57.8	57.7	1200-1219 bp	1.0	78.2