Hotspot

trnS-GCU-trnG-UCC

LSC · 8770-9493 · 724 bp

Back to Ehretia

Pi 0.0166

MarkerSeek score 64.80

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 724
variable_sites: 18
pi: 0.0166
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CCATCAACGGAAAGAGAGGG	TGAATCAAACCGAAAGACCCT	55.0	42.9	58.0	57.5	897-933 bp	1.0	69.5
trnS-GCU-trnG-UCC_p2	ATTAGCAATCCGCCGCTTTA	TGAATCAAACCGAAAGACCCT	45.0	42.9	58.0	57.5	842-878 bp	1.0	69.5
trnS-GCU-trnG-UCC_p3	ACGGAAAGAGAGGGATTCGA	TGAATCAAACCGAAAGACCCT	50.0	42.9	58.2	57.5	891-927 bp	1.0	68.5
trnS-GCU-trnG-UCC_p4	AGCCATCAACGGAAAGAGAG	TGAATCAAACCGAAAGACCCT	50.0	42.9	57.6	57.5	899-935 bp	1.0	66.6
trnS-GCU-trnG-UCC_p5	GCTTTAGTCCACTCAGCCAT	TGAATCAAACCGAAAGACCCT	50.0	42.9	57.6	57.5	828-864 bp	1.0	66.6