Hotspot

trnH-GUG-psbA

LSC · 106-379 · 274 bp

Back to Ehretia

Pi 0.0323

MarkerSeek score 63.30

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 274
variable_sites: 13
pi: 0.0323
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	CTGCCTTAATCCACTTGGCT	CCCTCTAGACTTAGCGGCTA	50.0	55.0	57.9	57.7	343-348 bp	1.0	81.1
trnH-GUG-psbA_p2	ACTGCCTTAATCCACTTGGC	CCCTCTAGACTTAGCGGCTA	50.0	55.0	58.2	57.7	344-349 bp	1.0	80.9
trnH-GUG-psbA_p3	CTGCCTTAATCCACTTGGCT	CCGTGCTAACCTTGGTATGG	50.0	55.0	57.9	58.3	394-399 bp	1.0	80.1
trnH-GUG-psbA_p4	ACTGCCTTAATCCACTTGGC	CCGTGCTAACCTTGGTATGG	50.0	55.0	58.2	58.3	395-400 bp	1.0	79.9
trnH-GUG-psbA_p5	CTGCCTTAATCCACTTGGCT	ACCGTGCTAACCTTGGTATG	50.0	50.0	57.9	57.6	395-400 bp	1.0	79.6