Hotspot

ndhF-rpl32

SSC · 131002-131340 · 339 bp

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Pi 0.146

MarkerSeek score 74.30

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 339
variable_sites: 73
pi: 0.146
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	ACACATTCCAACCAGTTCCC	AAAAGCTTTCAACGCTGCTC	50.0	45.0	58.0	57.9	900 bp	0.333333	52.3
ndhF-rpl32_p2	CACATTCCAACCAGTTCCCA	AAAAGCTTTCAACGCTGCTC	50.0	45.0	58.0	57.9	899 bp	0.333333	52.3
ndhF-rpl32_p3	AAGGGGAAATTGTGCGGATT	AAAAGCTTTCAACGCTGCTC	45.0	45.0	58.1	57.9	1178 bp	0.333333	51.8
ndhF-rpl32_p4	AGGGGAAATTGTGCGGATTT	AAAAGCTTTCAACGCTGCTC	45.0	45.0	58.1	57.9	1177 bp	0.333333	51.8
ndhF-rpl32_p5	AGGAAGTGGAACCAAGGGTA	AAAAGCTTTCAACGCTGCTC	50.0	45.0	57.9	57.9	486 bp	0.333333	51.3