Hotspot

trnL-UAG-ndhF

SSC · 127887-130395 · 2509 bp

Back to Edgeworthia

Pi 0.0362

MarkerSeek score 66.30

Primer available yes

Label trnL-UAG-ndhF

Feature Metadata

label_name: trnL-UAG-ndhF
feature_type: igs
region: SSC
length_bp: 2509
variable_sites: 133
pi: 0.0362
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-ndhF_p1	TTATAAGACGCTATGCCGCC	GGGAACTGGTTGGAATGTGT	50.0	50.0	57.9	58.0	2763-2878 bp	0.666667	70.7
trnL-UAG-ndhF_p2	TCCTAAGAGCAGCGTGTCTA	GGGAACTGGTTGGAATGTGT	50.0	50.0	57.9	58.0	2709-2824 bp	0.666667	70.4
trnL-UAG-ndhF_p3	AGCAGCGTGTCTACCAATTT	GGGAACTGGTTGGAATGTGT	45.0	50.0	57.8	58.0	2702-2817 bp	0.666667	69.9
trnL-UAG-ndhF_p4	TTATAAGACGCTATGCCGCC	TGGGAACTGGTTGGAATGTG	50.0	50.0	57.9	58.0	2764 bp	0.333333	52.7
trnL-UAG-ndhF_p5	TCCTAAGAGCAGCGTGTCTA	TGGGAACTGGTTGGAATGTG	50.0	50.0	57.9	58.0	2710 bp	0.333333	52.3