Hotspot

rpl36-rps8

LSC · 82204-82708 · 505 bp

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Pi 0.0212

MarkerSeek score 57.80

Primer available yes

Label rpl36-rps8

Feature Metadata

label_name: rpl36-rps8
feature_type: igs
region: LSC
length_bp: 505
variable_sites: 16
pi: 0.0212
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-rps8_p1	CCCTGAATCCACAAGTACCG	AAAACGGATAAGTCGACCCG	55.0	50.0	58.0	58.0	1117-1130 bp	1.0	81.9
rpl36-rps8_p2	CCTGAATCCACAAGTACCGG	AAAACGGATAAGTCGACCCG	55.0	50.0	58.0	58.0	1116-1129 bp	1.0	81.9
rpl36-rps8_p3	GAAATCACCCGACCCCTTAC	AAAACGGATAAGTCGACCCG	55.0	50.0	58.0	58.0	1085-1098 bp	1.0	81.9
rpl36-rps8_p4	AGAAATCACCCGACCCCTTA	AAAACGGATAAGTCGACCCG	50.0	50.0	58.0	58.0	1086-1099 bp	1.0	81.9
rpl36-rps8_p5	GTCCCTCTTCTTGTACCCCT	AAAACGGATAAGTCGACCCG	55.0	50.0	58.0	58.0	1133-1146 bp	1.0	81.7