Hotspot

psbN-psbH

LSC · 76853-76959 · 107 bp

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Pi 0.0249

MarkerSeek score 65.80

Primer available yes

Label psbN-psbH

Feature Metadata

label_name: psbN-psbH
feature_type: igs
region: LSC
length_bp: 107
variable_sites: 4
pi: 0.0249
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbN-psbH_p1	CGACTAAGGTTGCTGGTTCC	ATCCAGGGGCTACTTTACCA	55.0	50.0	58.6	57.7	238 bp	1.0	74.6
psbN-psbH_p2	CGACTAAGGTTGCTGGTTCC	TCCAGGGGCTACTTTACCAT	55.0	50.0	58.6	57.7	237 bp	1.0	74.6
psbN-psbH_p3	GACTAAGGTTGCTGGTTCCA	ATCCAGGGGCTACTTTACCA	50.0	50.0	57.4	57.7	237 bp	1.0	74.6
psbN-psbH_p4	GACTAAGGTTGCTGGTTCCA	TCCAGGGGCTACTTTACCAT	50.0	50.0	57.4	57.7	236 bp	1.0	74.6
psbN-psbH_p5	TAGTTGTTGAGAGGGTTGCC	TCCAGGGGCTACTTTACCAT	50.0	50.0	57.4	57.7	317 bp	1.0	74.3