Hotspot

psbT-psbN

LSC · 76639-76720 · 82 bp

Back to Edgeworthia

Pi 0.0325

MarkerSeek score 66.30

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 82
variable_sites: 4
pi: 0.0325
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	TCGAGAACCGCCTAAAGTTC	GATCCATTCGAGGAACACGG	50.0	55.0	57.6	58.4	143 bp	1.0	75.9
psbT-psbN_p2	GAGAACCGCCTAAAGTTCCA	GATCCATTCGAGGAACACGG	50.0	55.0	57.5	58.4	141 bp	1.0	75.1
psbT-psbN_p3	TCGAGAACCGCCTAAAGTTC	AGATCCATTCGAGGAACACG	50.0	50.0	57.6	57.4	144 bp	1.0	74.7
psbT-psbN_p4	TCGAGAACCGCCTAAAGTTC	GGCAACCCTCTCAACAACTA	50.0	50.0	57.6	57.4	166 bp	1.0	74.6
psbT-psbN_p5	GAGAACCGCCTAAAGTTCCA	GGCAACCCTCTCAACAACTA	50.0	50.0	57.5	57.4	164 bp	1.0	73.7