Hotspot

petA-psbJ

LSC · 66544-67331 · 788 bp

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Pi 0.0186

MarkerSeek score 70.60

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 788
variable_sites: 22
pi: 0.0186
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CCATTACGTGTTCAAGGCCT	GCCGATACTACTGGAAGGAT	50.0	50.0	58.2	56.2	1025-1052 bp	1.0	65.9
petA-psbJ_p2	CCATTACGTGTTCAAGGCCT	GCCGATACTACTGGAAGGATT	50.0	47.6	58.2	57.0	1025-1052 bp	1.0	64.0
petA-psbJ_p3	ACCCATTACGTGTTCAAGGC	GCCGATACTACTGGAAGGAT	50.0	50.0	58.5	56.2	1027-1054 bp	1.0	63.6
petA-psbJ_p4	CCATTACGTGTTCAAGGCCT	GCCGATACTACTGGAAGGATTC	50.0	50.0	58.2	58.1	1025-1052 bp	1.0	63.6
petA-psbJ_p5	ACGTGTTCAAGGCCTTTTGT	GCCGATACTACTGGAAGGAT	45.0	50.0	58.5	56.2	1020-1047 bp	1.0	63.1