Hotspot

psbI-trnS-GCU

LSC · 8720-8801 · 82 bp

Back to Edgeworthia

Pi 0.047

MarkerSeek score 81.20

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 82
variable_sites: 5
pi: 0.047
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 81.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	TTTCGCTCTTCATCTTCGGA	TGGACTAAAGCGTCGGATTG	45.0	50.0	57.0	58.0	213-220 bp	1.0	76.2
psbI-trnS-GCU_p2	TTTCGCTCTTCATCTTCGGA	GTGGACTAAAGCGTCGGATT	45.0	50.0	57.0	58.0	214-221 bp	1.0	76.1
psbI-trnS-GCU_p3	GTTTCGCTCTTCATCTTCGG	TGGACTAAAGCGTCGGATTG	50.0	50.0	56.9	58.0	214-221 bp	1.0	75.8
psbI-trnS-GCU_p4	TCGCTCTTCATCTTCGGATTC	TGGACTAAAGCGTCGGATTG	47.6	50.0	57.9	58.0	211-218 bp	1.0	75.8
psbI-trnS-GCU_p5	GTTTCGCTCTTCATCTTCGG	GTGGACTAAAGCGTCGGATT	50.0	50.0	56.9	58.0	215-222 bp	1.0	75.7