Hotspot

trnK-UUU-rps16

LSC · 4444-5118 · 675 bp

Back to Edgeworthia

Pi 0.0173
MarkerSeek score 67.00
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
675
variable_sites
17
pi
0.0173
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
67.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCGATGGTATGGACGAATCC TTTAAAGAAGGCGGGGGTTT 55.0 45.0 57.9 57.6 807-828 bp 1.0 77.9
trnK-UUU-rps16_p2 CCGATGGTATGGACGAATCC TTTTAAAGAAGGCGGGGGTT 55.0 45.0 57.9 57.6 808-829 bp 1.0 77.9
trnK-UUU-rps16_p3 CGATGGTATGGACGAATCCC TTTAAAGAAGGCGGGGGTTT 55.0 45.0 57.9 57.6 806-827 bp 1.0 77.9
trnK-UUU-rps16_p4 CGATGGTATGGACGAATCCC TTTTAAAGAAGGCGGGGGTT 55.0 45.0 57.9 57.6 807-828 bp 1.0 77.9
trnK-UUU-rps16_p5 CGATGGTATGGACGAATCCC CGGGGGTTTACGGAACTAAA 55.0 50.0 57.9 57.5 795-816 bp 1.0 77.3