Hotspot

ycf1

SSC · 122186-127252 · 5067 bp

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Pi 0.00118

MarkerSeek score 38.40

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5067
variable_sites: 6
pi: 0.00118
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 38.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCATCCAAAAATTGACTCCAGT	AATTATGGAACCTGCCCGTC	36.4	50.0	55.9	57.9	351 bp	1.0	47.2
ycf1_p2	TCATCCAAAAATTGACTCCAGT	TATGGAACCTGCCCGTCTAT	36.4	50.0	55.9	57.9	348 bp	1.0	46.8
ycf1_p3	TCATCCAAAAATTGACTCCAGT	TTATGGAACCTGCCCGTCTA	36.4	50.0	55.9	58.1	349 bp	1.0	46.5
ycf1_p4	TCATCCAAAAATTGACTCCAGT	AACCTGCCCGTCTATCTGTA	36.4	50.0	55.9	57.8	343 bp	1.0	46.4
ycf1_p5	TCATCCAAAAATTGACTCCAGT	ACCTGCCCGTCTATCTGTAA	36.4	50.0	55.9	57.8	342 bp	1.0	46.4