Hotspot

ndhD

SSC · 114018-115520 · 1503 bp

Back to Eclipta

Pi 0.000665

MarkerSeek score 47.70

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 1
pi: 0.000665
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	TGCCAACCAACTCTTAAAAA	GGCACAGGTTTTTCTGGTCT	35.0	50.0	53.5	58.3	1583 bp	1.0	41.5
ndhD_p2	TTGCCAACCAACTCTTAAAA	GGCACAGGTTTTTCTGGTCT	35.0	50.0	53.5	58.3	1584 bp	1.0	41.5
ndhD_p3	TTTGCCAACCAACTCTTAAA	GGCACAGGTTTTTCTGGTCT	35.0	50.0	53.5	58.3	1585 bp	1.0	41.5
ndhD_p4	TTTTGCCAACCAACTCTTAA	GGCACAGGTTTTTCTGGTCT	35.0	50.0	53.5	58.3	1586 bp	1.0	41.5
ndhD_p5	TTTTTGCCAACCAACTCTTA	GGCACAGGTTTTTCTGGTCT	35.0	50.0	53.5	58.3	1587 bp	1.0	41.5