Hotspot

ndhA

SSC · 118124-120241 · 2118 bp

Back to Eclipta

Pi 0.00142

MarkerSeek score 58.30

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2118
variable_sites: 3
pi: 0.00142
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TGTTTTGGACAAGTCGTGAA	GGCCGCCGGGATTTATTAAT	40.0	50.0	55.8	58.5	2273 bp	1.0	58.4
ndhA_p2	TTGTTTTGGACAAGTCGTGA	GGCCGCCGGGATTTATTAAT	40.0	50.0	55.8	58.5	2274 bp	1.0	58.4
ndhA_p3	TTGTTTTGGACAAGTCGTGAA	GGCCGCCGGGATTTATTAAT	38.1	50.0	56.5	58.5	2274 bp	1.0	56.6
ndhA_p4	TGTTTTGGACAAGTCGTGAAT	GGCCGCCGGGATTTATTAAT	38.1	50.0	56.3	58.5	2273 bp	1.0	54.9
ndhA_p5	TGTTTTGGACAAGTCGTGAA	GGCCGCCGGGATTTATTAA	40.0	52.6	55.8	58.0	2273 bp	1.0	53.9