Hotspot

rpoA-rps11

LSC · 78113-78192 · 80 bp

Back to Eclipta

Pi 0.0125

MarkerSeek score 59.70

Primer available yes

Label rpoA-rps11

Feature Metadata

label_name: rpoA-rps11
feature_type: igs
region: LSC
length_bp: 80
variable_sites: 1
pi: 0.0125
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA-rps11_p1	ACTTCCACTGTAGTGTCCGA	AAGAGACGCAGCATTACGAG	50.0	50.0	58.0	58.1	244 bp	1.0	79.6
rpoA-rps11_p2	TTCCACTGTAGTGTCCGAGT	AAGAGACGCAGCATTACGAG	50.0	50.0	58.0	58.1	242 bp	1.0	79.6
rpoA-rps11_p3	ACTTCCACTGTAGTGTCCGA	GAAGAGACGCAGCATTACGA	50.0	50.0	58.0	58.1	245 bp	1.0	79.5
rpoA-rps11_p4	TTCCACTGTAGTGTCCGAGT	GAAGAGACGCAGCATTACGA	50.0	50.0	58.0	58.1	243 bp	1.0	79.5
rpoA-rps11_p5	GCTTACTGTCTGCTGCTGAT	AAGAGACGCAGCATTACGAG	50.0	50.0	58.0	58.1	271 bp	1.0	79.4