Hotspot

atpB-rbcL

LSC · 53431-54198 · 768 bp

Back to Eclipta

Pi 0.00131

MarkerSeek score 60.30

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 768
variable_sites: 1
pi: 0.00131
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	CTGGCGGAAAAGCTACATCT	CACCAGCTTTGAATCCAACAC	50.0	47.6	58.0	58.0	923-929 bp	1.0	72.2
atpB-rbcL_p2	CTGGCGGAAAAGCTACATCT	ACCAGCTTTGAATCCAACACT	50.0	42.9	58.0	58.1	922-928 bp	1.0	72.0
atpB-rbcL_p3	GGCAACCCCAGAACTAGAAG	CACCAGCTTTGAATCCAACAC	55.0	47.6	57.9	58.0	850-856 bp	1.0	71.6
atpB-rbcL_p4	GATACGCCCCAGGTTCTTTT	CACCAGCTTTGAATCCAACAC	50.0	47.6	57.9	58.0	880-886 bp	1.0	71.5
atpB-rbcL_p5	GGCAACCCCAGAACTAGAAG	ACCAGCTTTGAATCCAACACT	55.0	42.9	57.9	58.1	849-855 bp	1.0	71.3