Hotspot

secA

LSC · 31539-34172 · 2634 bp

Back to Ecklonia

Pi 0.00861

MarkerSeek score 46.90

Primer available yes

Label secA

Feature Metadata

label_name: secA
feature_type: gene
region: LSC
length_bp: 2634
variable_sites: 34
pi: 0.00861
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
secA_p1	GCACGAAAACCAGAAACAGC	TTACACACTATTAAAGAATCACTT	50.0	25.0	58.5	52.1	2999 bp	0.666667	27.3
secA_p2	GCACGAAAACCAGAAACAGC	TTTACACACTATTAAAGAATCACT	50.0	25.0	58.5	52.1	3000 bp	0.666667	27.3
secA_p3	AGCACGAAAACCAGAAACAG	TTACACACTATTAAAGAATCACTT	45.0	25.0	56.9	52.1	3000 bp	0.666667	27.3
secA_p4	CACGAAAACCAGAAACAGCT	TTACACACTATTAAAGAATCACTT	45.0	25.0	56.9	52.1	2998 bp	0.666667	27.3
secA_p5	CACGAAAACCAGAAACAGCT	TTTACACACTATTAAAGAATCACT	45.0	25.0	56.9	52.1	2999 bp	0.666667	27.3