Hotspot

psaL

LSC · 79857-80294 · 438 bp

Back to Ecklonia

Pi 0.0183

MarkerSeek score 52.10

Primer available yes

Label psaL

Feature Metadata

label_name: psaL
feature_type: gene
region: LSC
length_bp: 438
variable_sites: 12
pi: 0.0183
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaL_p1	TTATACCCACTGCCAACGTC	GCGGGATTAGGGGTTTCATT	50.0	50.0	57.6	57.9	1377-1383 bp	1.0	78.5
psaL_p2	TTATACCCACTGCCAACGTC	GAGTTCAAGCGGGATTAGGG	50.0	55.0	57.6	58.0	1391 bp	0.666667	64.3
psaL_p3	TTATACCCACTGCCAACGTC	GGAGTTCAAGCGGGATTAGG	50.0	55.0	57.6	58.0	1392 bp	0.666667	64.3
psaL_p4	AGATGCAAGTACAAAGGCGA	GAGTTCAAGCGGGATTAGGG	45.0	55.0	57.5	58.0	969 bp	0.666667	63.8
psaL_p5	AGATGCAAGTACAAAGGCGA	GGAGTTCAAGCGGGATTAGG	45.0	55.0	57.5	58.0	970 bp	0.666667	63.8