Hotspot

cp74

LSC · 63311-64879 · 1569 bp

Back to Ecklonia

Pi 0.0127

MarkerSeek score 52.00

Primer available yes

Label cp74

Feature Metadata

label_name: cp74
feature_type: gene
region: LSC
length_bp: 1569
variable_sites: 30
pi: 0.0127
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cp74_p1	TGCGGTAAAGAAGGCTTACC	GATCCTAATGTTGGCGGGTT	50.0	50.0	57.9	57.9	2510-2531 bp	1.0	80.8
cp74_p2	TTTGGCCAGTATCAGCTTGG	GATCCTAATGTTGGCGGGTT	50.0	50.0	58.2	57.9	2293-2314 bp	1.0	80.6
cp74_p3	TGGGCTGGTCGTAGTTATCT	GATCCTAATGTTGGCGGGTT	50.0	50.0	57.8	57.9	2402-2423 bp	1.0	80.5
cp74_p4	TGCGGTAAAGAAGGCTTACC	GGCAAATAGAGGTCGAGGAC	50.0	55.0	57.9	57.8	2742-2763 bp	1.0	79.3
cp74_p5	TGCGGTAAAGAAGGCTTACC	CCGATTTTATCCCCTGACCC	50.0	55.0	57.9	57.7	2810-2831 bp	1.0	79.0