Hotspot

psaL-rbcR

LSC · 80295-80346 · 52 bp

Back to Ecklonia

Pi 0.0641

MarkerSeek score 75.80

Primer available yes

Label psaL-rbcR

Feature Metadata

label_name: psaL-rbcR
feature_type: igs
region: LSC
length_bp: 52
variable_sites: 5
pi: 0.0641
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaL-rbcR_p1	TGGAGGCGTTAGTTTTGCTT	CCCATAAATCACAATTATCGGGG	45.0	43.5	58.0	57.4	196 bp	1.0	52.7
psaL-rbcR_p2	TTGGAGGCGTTAGTTTTGCT	CCCATAAATCACAATTATCGGGG	45.0	43.5	58.0	57.4	197 bp	1.0	52.7
psaL-rbcR_p3	TGGAGGCGTTAGTTTTGCTT	ACCCATAAATCACAATTATCGGG	45.0	39.1	58.0	56.7	197 bp	1.0	47.4
psaL-rbcR_p4	TTGGAGGCGTTAGTTTTGCT	ACCCATAAATCACAATTATCGGG	45.0	39.1	58.0	56.7	198 bp	1.0	47.4
psaL-rbcR_p5	TTTGGAGGCGTTAGTTTTGC	CCCATAAATCACAATTATCGGGG	45.0	43.5	57.2	57.4	198 bp	1.0	46.6