Hotspot

rbcS-trnR

LSC · 51995-52362 · 368 bp

Back to Ecklonia

Pi 0.0199

MarkerSeek score 66.20

Primer available yes

Label rbcS-trnR

Feature Metadata

label_name: rbcS-trnR
feature_type: igs
region: LSC
length_bp: 368
variable_sites: 11
pi: 0.0199
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcS-trnR_p1	GACCTTCAAACGAACCTGGT	CAGGCCCAGAAGGAATTGAA	50.0	50.0	58.0	57.8	557-579 bp	1.0	79.8
rbcS-trnR_p2	GACCTTCAAACGAACCTGGT	AGGCCCAGAAGGAATTGAAC	50.0	50.0	58.0	57.8	556-578 bp	1.0	79.8
rbcS-trnR_p3	GACCTTCAAACGAACCTGGT	GCCCAGAAGGAATTGAACCT	50.0	50.0	58.0	57.8	554-576 bp	1.0	79.8
rbcS-trnR_p4	GACCTTCAAACGAACCTGGT	TATTTGAGCAGGCCCAGAAG	50.0	50.0	58.0	57.6	565-587 bp	1.0	78.1
rbcS-trnR_p5	AACGACCTTCAAACGAACCT	CAGGCCCAGAAGGAATTGAA	45.0	50.0	57.7	57.8	560-582 bp	1.0	77.5