Hotspot

ycf1

SSC · 119682-125093 · 5412 bp

Pi 0.0137

MarkerSeek score 46.60

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5412
variable_sites: 74
pi: 0.0137
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TTTTTGTGGGTGCAGGTGAA	GAAACCTTGGCGCAGATCTA	45.0	50.0	58.4	58.0	470 bp	0.5	56.3
ycf1_p2	TTTTTGTGGGTGCAGGTGAA	AGTTTGGGGAATGGAAGCTG	45.0	50.0	58.4	58.1	392 bp	0.5	55.9
ycf1_p3	TTTTTGTGGGTGCAGGTGAA	CAGTTTGGGGAATGGAAGCT	45.0	50.0	58.4	58.1	393 bp	0.5	55.9
ycf1_p4	TTTTTGTGGGTGCAGGTGAA	GTTTGGGGAATGGAAGCTGA	45.0	50.0	58.4	58.1	391 bp	0.5	55.9
ycf1_p5	TTTTTGTGGGTGCAGGTGAA	TTGGGGAATGGAAGCTGAAC	45.0	50.0	58.4	58.1	389 bp	0.5	55.9