Hotspot

trnR-ACG-trnN-GUU

IRb · 104674-105267 · 594 bp

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Pi 0.0135
MarkerSeek score 66.40
Primer available yes
Label trnR-ACG-trnN-GUU

Feature Metadata

label_name
trnR-ACG-trnN-GUU
feature_type
igs
region
IRb
length_bp
594
variable_sites
8
pi
0.0135
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-ACG-trnN-GUU_p1 CAGAGGATTAGAGCACGTGG CCGTTGTTCCTCAGTAGCTC 55.0 55.0 57.8 58.0 735 bp 1.0 79.1
trnR-ACG-trnN-GUU_p2 GAACAGATCCAGTGGAGACG CCGTTGTTCCTCAGTAGCTC 55.0 55.0 57.7 58.0 772 bp 1.0 78.5
trnR-ACG-trnN-GUU_p3 CAGAGGATTAGAGCACGTGG TGGGTTGAGAACGGGAATTG 55.0 50.0 57.8 58.1 776 bp 1.0 78.3
trnR-ACG-trnN-GUU_p4 CAGAGGATTAGAGCACGTGG TGGTCGTAGGTTCGAATCCT 55.0 50.0 57.8 58.2 686 bp 1.0 78.0
trnR-ACG-trnN-GUU_p5 GAACAGATCCAGTGGAGACG TGGGTTGAGAACGGGAATTG 55.0 50.0 57.7 58.1 813 bp 1.0 77.7