Hotspot

rpl33-rps18

LSC · 65430-65577 · 148 bp

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Pi 0.0141
MarkerSeek score 64.50
Primer available yes
Label rpl33-rps18

Feature Metadata

label_name
rpl33-rps18
feature_type
igs
region
LSC
length_bp
148
variable_sites
2
pi
0.0141
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl33-rps18_p1 AAGAACCGACAGAATACGCC CAATTCGATCCCCCGATTCA 50.0 50.0 58.0 57.7 320-326 bp 1.0 79.9
rpl33-rps18_p2 AAGAACCGACAGAATACGCC GGGCAAACGCTTACGAAAAG 50.0 50.0 58.0 58.3 295-301 bp 1.0 79.6
rpl33-rps18_p3 AAGAACCGACAGAATACGCC GGCAAACGCTTACGAAAAGT 50.0 45.0 58.0 57.6 294-300 bp 1.0 79.0
rpl33-rps18_p4 AAGAACCGACAGAATACGCC TCAATTCGATCCCCCGATTC 50.0 50.0 58.0 57.5 321-327 bp 1.0 77.6
rpl33-rps18_p5 AAGAACCGACAGAATACGCC TTCGATCCCCCGATTCAATC 50.0 50.0 58.0 57.5 317-323 bp 1.0 77.6