Hotspot

psbE-petL

LSC · 62456-63544 · 1089 bp

Back to Echium

Pi 0.025
MarkerSeek score 68.70
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1089
variable_sites
27
pi
0.025
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
68.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 AGAACGTTCTCCTGTGCTTC GCCGCTAGTAGAAAACCGAA 50.0 50.0 57.8 58.0 1157-1190 bp 1.0 81.1
psbE-petL_p2 AGAACGTTCTCCTGTGCTTC AAGCCGCTAGTAGAAAACCG 50.0 50.0 57.8 58.0 1159-1192 bp 1.0 81.0
psbE-petL_p3 GTTCTCCTGTGCTTCCAGAC GCCGCTAGTAGAAAACCGAA 55.0 50.0 58.2 58.0 1152-1185 bp 1.0 80.7
psbE-petL_p4 GTTCTCCTGTGCTTCCAGAC AAGCCGCTAGTAGAAAACCG 55.0 50.0 58.2 58.0 1154-1187 bp 1.0 80.7
psbE-petL_p5 TCTCCTGTGCTTCCAGACAT GCCGCTAGTAGAAAACCGAA 50.0 50.0 58.4 58.0 1150-1183 bp 1.0 79.5