Hotspot

ndhC-trnV-UAC

LSC · 49499-50130 · 632 bp

Back to Echium

Pi 0.00949
MarkerSeek score 64.20
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
632
variable_sites
6
pi
0.00949
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TCTGGCCCTTTGTTAATCGG AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.9 57.8 781 bp 1.0 78.1
ndhC-trnV-UAC_p2 CGGGGCTAAAACTCCTGAAA AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.8 57.8 764 bp 1.0 77.5
ndhC-trnV-UAC_p3 TCTGGCCCTTTGTTAATCGG AGTGCATGTTGGGTCTTTGA 50.0 45.0 57.9 57.6 848 bp 1.0 76.7
ndhC-trnV-UAC_p4 CGGGGCTAAAACTCCTGAAA AGTGCATGTTGGGTCTTTGA 50.0 45.0 57.8 57.6 831 bp 1.0 76.1
ndhC-trnV-UAC_p5 ATCGGGGCTAAAACTCCTGA AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.4 57.8 766 bp 1.0 75.7