Hotspot

trnF-GAA-ndhJ

LSC · 47174-47801 · 628 bp

Back to Echium

Pi 0.00914
MarkerSeek score 66.30
Primer available yes
Label trnF-GAA-ndhJ

Feature Metadata

label_name
trnF-GAA-ndhJ
feature_type
igs
region
LSC
length_bp
628
variable_sites
5
pi
0.00914
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.87
markerseek_score
66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnF-GAA-ndhJ_p1 GGGATAGCTCAGCTGGTAGA ATCATCCACGCTTGAAACGT 55.0 45.0 58.0 58.2 749-805 bp 1.0 83.0
trnF-GAA-ndhJ_p2 GGGATAGCTCAGCTGGTAGA GATAGGATGGCCCCTACGTA 55.0 55.0 58.0 57.8 709-765 bp 1.0 82.7
trnF-GAA-ndhJ_p3 CCTCGTGTCACCAGTTCAAA ATCATCCACGCTTGAAACGT 50.0 45.0 58.1 58.2 713-769 bp 1.0 82.6
trnF-GAA-ndhJ_p4 GGGATAGCTCAGCTGGTAGA GTTGGATAGGATGGCCCCTA 55.0 55.0 58.0 58.3 713-769 bp 1.0 82.5
trnF-GAA-ndhJ_p5 GGGATAGCTCAGCTGGTAGA TTGGATAGGATGGCCCCTAC 55.0 55.0 58.0 58.3 712-768 bp 1.0 82.5