Hotspot

rps4-trnT-UGU

LSC · 45149-45494 · 346 bp

Back to Echium

Pi 0.0405
MarkerSeek score 68.40
Primer available yes
Label rps4-trnT-UGU

Feature Metadata

label_name
rps4-trnT-UGU
feature_type
igs
region
LSC
length_bp
346
variable_sites
14
pi
0.0405
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps4-trnT-UGU_p1 TAATCCTGGTAAAGCCCCCA CTCAGAGGTTAGAGCATCGC 50.0 55.0 58.0 57.9 472-479 bp 1.0 81.2
rps4-trnT-UGU_p2 TCTAAGATCACTCCCGGGTC CTCAGAGGTTAGAGCATCGC 55.0 55.0 57.9 57.9 508-515 bp 1.0 80.6
rps4-trnT-UGU_p3 AAGATCACTCCCGGGTCTAG CTCAGAGGTTAGAGCATCGC 55.0 55.0 57.9 57.9 505-512 bp 1.0 80.5
rps4-trnT-UGU_p4 CTAAGATCACTCCCGGGTCT CTCAGAGGTTAGAGCATCGC 55.0 55.0 57.9 57.9 507-514 bp 1.0 80.5
rps4-trnT-UGU_p5 TAATCCTGGTAAAGCCCCCA ATGGTCATCGGTTCGATTCC 50.0 50.0 58.0 57.8 440-447 bp 1.0 80.5