Hotspot

pafI-trnS-GGA

LSC · 43284-44188 · 905 bp

Back to Echium

Pi 0.0167
MarkerSeek score 66.00
Primer available yes
Label pafI-trnS-GGA

Feature Metadata

label_name
pafI-trnS-GGA
feature_type
igs
region
LSC
length_bp
905
variable_sites
15
pi
0.0167
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
pafI-trnS-GGA_p1 TTCTCCTGAAGTTGTCGGAA AAGGTTCGGAAAGAGAGGGA 45.0 50.0 56.4 58.0 1092-1094 bp 1.0 68.8
pafI-trnS-GGA_p2 TTTCTCCTGAAGTTGTCGGA AAGGTTCGGAAAGAGAGGGA 45.0 50.0 56.4 58.0 1093-1095 bp 1.0 68.8
pafI-trnS-GGA_p3 TTCTCCTGAAGTTGTCGGAA CCCTCGGTAAACAAAAGCCT 45.0 50.0 56.4 58.1 1066-1068 bp 1.0 68.3
pafI-trnS-GGA_p4 TTTCTCCTGAAGTTGTCGGA CCCTCGGTAAACAAAAGCCT 45.0 50.0 56.4 58.1 1067-1069 bp 1.0 68.3
pafI-trnS-GGA_p5 TTCTCCTGAAGTTGTCGGAA TCGGAAAGAGAGGGATTCGA 45.0 50.0 56.4 57.9 1087-1089 bp 1.0 67.9