Hotspot

trnT-GGU-psbD

LSC · 30430-31600 · 1171 bp

Back to Echium

Pi 0.0146
MarkerSeek score 68.00
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1171
variable_sites
17
pi
0.0146
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTGGTAGAGTAACGCCATGG CCGCAACCAGTCATCCATAA 55.0 50.0 58.1 58.0 1294-1301 bp 1.0 80.9
trnT-GGU-psbD_p2 TCAGTGGTAGAGTAACGCCA CCGCAACCAGTCATCCATAA 50.0 50.0 58.1 58.0 1297-1304 bp 1.0 80.7
trnT-GGU-psbD_p3 GTGGTAGAGTAACGCCATGG GGACCAGCCTACAAAAACGA 55.0 50.0 58.1 58.1 1324-1331 bp 1.0 80.2
trnT-GGU-psbD_p4 TCAGTGGTAGAGTAACGCCA GGACCAGCCTACAAAAACGA 50.0 50.0 58.1 58.1 1327-1334 bp 1.0 80.0
trnT-GGU-psbD_p5 GTGGTAGAGTAACGCCATGG ACAAAAACGAAACGGTCCCT 55.0 45.0 58.1 58.2 1314-1321 bp 1.0 79.2