Hotspot

atpF-atpH

LSC · 12110-12474 · 365 bp

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Pi 0.0537
MarkerSeek score 85.80
Primer available yes
Label atpF-atpH

Feature Metadata

label_name
atpF-atpH
feature_type
igs
region
LSC
length_bp
365
variable_sites
19
pi
0.0537
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
85.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpF-atpH_p1 TGGCCCAAAGAAACGAAAGA GGCTGGTTGTAGCATTAGCA 45.0 50.0 57.9 58.2 450-468 bp 1.0 82.7
atpF-atpH_p2 GTGGCCCAAAGAAACGAAAG GGCTGGTTGTAGCATTAGCA 50.0 50.0 57.9 58.2 451-469 bp 1.0 82.1
atpF-atpH_p3 TGGCCCAAAGAAACGAAAGA TGGGCTGGTTGTAGCATTAG 45.0 50.0 57.9 57.6 452-470 bp 1.0 81.4
atpF-atpH_p4 AGTGGCCCAAAGAAACGAAA GGCTGGTTGTAGCATTAGCA 45.0 50.0 58.2 58.2 452-470 bp 1.0 81.4
atpF-atpH_p5 GTGGCCCAAAGAAACGAAAG TGGGCTGGTTGTAGCATTAG 50.0 50.0 57.9 57.6 453-471 bp 1.0 80.8