Hotspot

trnH-GUG-psbA

LSC · 77-345 · 269 bp

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Pi 0.0856
MarkerSeek score 85.20
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
269
variable_sites
19
pi
0.0856
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.83
markerseek_score
85.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG CCGTGCTAACCTTGGTATGG 55.0 55.0 58.1 58.3 341-388 bp 1.0 84.4
trnH-GUG-psbA_p2 CAATCCACTGCCTTGATCCA CCGTGCTAACCTTGGTATGG 50.0 55.0 57.9 58.3 355-402 bp 1.0 84.3
trnH-GUG-psbA_p3 AATCCACTGCCTTGATCCAC CCGTGCTAACCTTGGTATGG 50.0 55.0 57.9 58.3 354-401 bp 1.0 84.3
trnH-GUG-psbA_p4 ACAATCCACTGCCTTGATCC CCGTGCTAACCTTGGTATGG 50.0 55.0 57.9 58.3 356-403 bp 1.0 84.2
trnH-GUG-psbA_p5 GGAATCACAATCCACTGCCT CCGTGCTAACCTTGGTATGG 50.0 55.0 57.9 58.3 362-409 bp 1.0 84.2