Hotspot

ycf22

Genome · 46980-47651 · 672 bp

Back to Echinothamnion

Pi 0.0952

MarkerSeek score 78.20

Primer available yes

Label ycf22

Feature Metadata

label_name: ycf22
feature_type: gene
region: Genome
length_bp: 672
variable_sites: 64
pi: 0.0952
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf22_p1	GGAGCAGATTTAGTTGAAGTTGG	AGCCATGCCAAAGTTTCTGA	43.5	45.0	57.7	58.0	1725 bp	0.5	33.6
ycf22_p2	GGAGCAGATTTAGTTGAAGTTGG	TCACCATGCAATTCAACCCA	43.5	45.0	57.7	58.0	1595 bp	0.5	33.5
ycf22_p3	GGAGCAGATTTAGTTGAAGTTGG	CAATAGCTTCGCCTTGTCCT	43.5	50.0	57.7	58.0	1864 bp	0.5	33.3
ycf22_p4	GGAGCAGATTTAGTTGAAGTTGG	AGCTTTGCGATATCCACCTG	43.5	50.0	57.7	58.0	1749 bp	0.5	33.3
ycf22_p5	GGAGCAGATTTAGTTGAAGTTGG	TTCAGCTTCAATACCAGCCC	43.5	50.0	57.7	57.9	1839 bp	0.5	32.6