Hotspot

syfB

Genome · 156441-158459 · 2019 bp

Back to Echinothamnion

Pi 0.129

MarkerSeek score 78.20

Primer available yes

Label syfB

Feature Metadata

label_name: syfB
feature_type: gene
region: Genome
length_bp: 2019
variable_sites: 260
pi: 0.129
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
syfB_p1	TGCCACAGAAATATACCGCC	CGAATCCTCCTACCCCAGAT	50.0	55.0	58.0	58.0	2765-2774 bp	1.0	87.8
syfB_p2	TCGGTAAGAGCGTACCAGAT	CGAATCCTCCTACCCCAGAT	50.0	55.0	58.0	58.0	2717-2726 bp	1.0	87.7
syfB_p3	AACCAAACCCGGCTTATGTT	CGAATCCTCCTACCCCAGAT	45.0	55.0	58.0	58.0	2511 bp	0.5	59.8
syfB_p4	ACCAAACCCGGCTTATGTTT	CGAATCCTCCTACCCCAGAT	45.0	55.0	58.0	58.0	2510 bp	0.5	59.8
syfB_p5	ACCAAACCCGGCTTATGTTT	CGTGATGGTAGTACAGGCAC	45.0	55.0	58.0	58.1	2366 bp	0.5	59.3