Hotspot

trnS.2-trnM.3

Genome · 140281-140302 · 22 bp

Back to Echinothamnion

Pi 0.227

MarkerSeek score 78.60

Primer available yes

Label trnS.2-trnM.3

Feature Metadata

label_name: trnS.2-trnM.3
feature_type: igs
region: Genome
length_bp: 22
variable_sites: 5
pi: 0.227
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS.2-trnM.3_p1	TAGCAATCTGCCGCTTTCAA	GACTCATAAGCCCAAGGTCG	45.0	55.0	58.2	58.1	105 bp	1.0	81.9
trnS.2-trnM.3_p2	TTAGCAATCTGCCGCTTTCA	GACTCATAAGCCCAAGGTCG	45.0	55.0	58.2	58.1	106 bp	1.0	81.9
trnS.2-trnM.3_p3	AGATTAGCAATCTGCCGCTT	GACTCATAAGCCCAAGGTCG	45.0	55.0	57.7	58.1	109 bp	1.0	80.5
trnS.2-trnM.3_p4	TAGCAATCTGCCGCTTTCAA	GGACTCATAAGCCCAAGGTC	45.0	55.0	58.2	57.7	106 bp	1.0	79.7
trnS.2-trnM.3_p5	TTAGCAATCTGCCGCTTTCA	GGACTCATAAGCCCAAGGTC	45.0	55.0	58.2	57.7	107 bp	1.0	79.6