Hotspot

pbsA-bas1

Genome · 104244-104388 · 145 bp

Back to Echinothamnion

Pi 0.148

MarkerSeek score 78.20

Primer available yes

Label pbsA-bas1

Feature Metadata

label_name: pbsA-bas1
feature_type: igs
region: Genome
length_bp: 145
variable_sites: 20
pi: 0.148
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 78.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
pbsA-bas1_p1	AGTTGTTCCTTCACGCAACT	GTCTGGTGGTGTTGGTGATT	45.0	50.0	58.0	58.0	506 bp	0.5	59.5
pbsA-bas1_p2	GTTGTTCCTTCACGCAACTG	GTCTGGTGGTGTTGGTGATT	50.0	50.0	57.9	58.0	505 bp	0.5	59.0
pbsA-bas1_p3	AGTTGTTCCTTCACGCAACT	GCAACCTGGTCAATCTGCTA	45.0	50.0	58.0	57.9	254 bp	0.5	58.8
pbsA-bas1_p4	AGTTGTTCCTTCACGCAACT	AATCCTGATGAAGTGTGCCC	45.0	50.0	58.0	57.9	283 bp	0.5	58.6
pbsA-bas1_p5	AGTTGTTCCTTCACGCAACT	TGGTTCAAATCCATTCCCCG	45.0	50.0	58.0	58.2	928 bp	0.5	58.5