Hotspot

trnP-ycf52

Genome · 7156-7307 · 152 bp

Back to Echinothamnion

Pi 0.248

MarkerSeek score 77.80

Primer available yes

Label trnP-ycf52

Feature Metadata

label_name: trnP-ycf52
feature_type: igs
region: Genome
length_bp: 152
variable_sites: 35
pi: 0.248
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 77.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-ycf52_p1	GCTACCAAGCTGCGCTATAT	CGGCTTCATGATAGATCCGG	50.0	55.0	57.9	58.0	231 bp	0.5	59.3
trnP-ycf52_p2	GCTACCAAGCTGCGCTATAT	ACTCAGTCGGTTGGGTAAGA	50.0	50.0	57.9	58.0	532 bp	0.5	59.3
trnP-ycf52_p3	GCTACCAAGCTGCGCTATAT	TTTGTGACTCAGTCGGTTGG	50.0	50.0	57.9	58.1	538 bp	0.5	58.9
trnP-ycf52_p4	GCTACCAAGCTGCGCTATAT	GTCGGTTGGGTAAGAAGACC	50.0	55.0	57.9	57.9	527 bp	0.5	58.6
trnP-ycf52_p5	GCTACCAAGCTGCGCTATAT	TTCAAGGACAAGGCTTAGGC	50.0	50.0	57.9	57.8	352 bp	0.5	57.7