Hotspot

trnE-UUC-rpoB

LSC · 12127-12984 · 858 bp

Back to Echinops

Pi 0.00932

MarkerSeek score 67.90

Primer available yes

Label trnE-UUC-rpoB

Feature Metadata

label_name: trnE-UUC-rpoB
feature_type: igs
region: LSC
length_bp: 858
variable_sites: 12
pi: 0.00932
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-rpoB_p1	CTTGAACCCTCGTTGTCTCC	AGTTCTTCTGTCAAACCCTGA	55.0	42.9	58.2	56.8	998 bp	1.0	63.5
trnE-UUC-rpoB_p2	CTTGAACCCTCGTTGTCTCC	TCAAACCCTGATCAATGAACCT	55.0	40.9	58.2	57.6	988 bp	1.0	62.2
trnE-UUC-rpoB_p3	AAACCTCTGGACCATAGGGG	AGTTCTTCTGTCAAACCCTGA	55.0	42.9	58.4	56.8	959 bp	1.0	62.1
trnE-UUC-rpoB_p4	CTTGAACCCTCGTTGTCTCC	TCTTCTGTCAAACCCTGATCA	55.0	42.9	58.2	56.6	995 bp	1.0	61.9
trnE-UUC-rpoB_p5	CGTTGTCTCCGTGAAAGAGA	AGTTCTTCTGTCAAACCCTGA	50.0	42.9	57.6	56.8	988 bp	1.0	61.9