Hotspot

psbI-trnS-GCU

LSC · 8400-8533 · 134 bp

Back to Echinops

Pi 0.0201

MarkerSeek score 65.90

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 134
variable_sites: 4
pi: 0.0201
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	CGTAATCCTGGACGTGAAGA	ATTGGGAGAGATGGCTGAGT	50.0	50.0	57.1	58.1	249-250 bp	1.0	73.3
psbI-trnS-GCU_p2	CGTAATCCTGGACGTGAAGA	CGAGTTATTCGTACCGAGGG	50.0	55.0	57.1	57.6	197-198 bp	1.0	71.4
psbI-trnS-GCU_p3	CGTAATCCTGGACGTGAAGAA	ATTGGGAGAGATGGCTGAGT	47.6	50.0	57.8	58.1	249-250 bp	1.0	70.9
psbI-trnS-GCU_p4	CCTATCCAATGATCCAGGACG	ATTGGGAGAGATGGCTGAGT	52.4	50.0	57.7	58.1	268-269 bp	1.0	70.0
psbI-trnS-GCU_p5	CGTAATCCTGGACGTGAAGA	GATGGCTGAGTGGACGAAAG	50.0	55.0	57.1	58.6	240-241 bp	1.0	69.1