Hotspot

ndhF-rpl32

SSC · 106784-107574 · 791 bp

Back to Echinochloa

Pi 0.00313

MarkerSeek score 48.00

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 791
variable_sites: 7
pi: 0.00313
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GGAAGTGGGAGAAGAGGGAT	TGACATCGAAGTGCGTTTTT	55.0	40.0	58.1	56.6	870-896 bp	1.0	68.9
ndhF-rpl32_p2	GGAAGTGGGAGAAGAGGGAT	TTGACATCGAAGTGCGTTTT	55.0	40.0	58.1	56.6	871-897 bp	1.0	68.9
ndhF-rpl32_p3	GGAAGTGGGAGAAGAGGGAT	TTTGACATCGAAGTGCGTTT	55.0	40.0	58.1	56.6	872-898 bp	1.0	68.9
ndhF-rpl32_p4	GGAAGTGGGAGAAGAGGGAT	TTTTGACATCGAAGTGCGTT	55.0	40.0	58.1	56.6	873-899 bp	1.0	68.9
ndhF-rpl32_p5	GGAAGTGGGAGAAGAGGGAT	TTTTTGACATCGAAGTGCGT	55.0	40.0	58.1	56.6	874-900 bp	1.0	68.9