Hotspot

petA-psbJ

LSC · 61884-62799 · 916 bp

Back to Echinochloa

Pi 0.00478

MarkerSeek score 41.10

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 916
variable_sites: 11
pi: 0.00478
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCCTTTTGTTCTTCTTCGC	TCTGGATTGGGTTCATCTCT	50.0	45.0	57.9	55.5	811-1042 bp	1.0	64.6
petA-psbJ_p2	GGCCTTTTGTTCTTCTTCGC	TTCTGGATTGGGTTCATCTCT	50.0	42.9	57.9	56.3	812-1043 bp	1.0	63.1
petA-psbJ_p3	CGTCCAAGGCCTTTTGTTCT	TCTGGATTGGGTTCATCTCT	50.0	45.0	58.7	55.5	818-1049 bp	1.0	59.5
petA-psbJ_p4	GGCCTTTTGTTCTTCTTCGC	ATTCTGGATTGGGTTCATCTCT	50.0	40.9	57.9	56.9	813-1044 bp	1.0	59.3
petA-psbJ_p5	GCCTTTTGTTCTTCTTCGCA	TCTGGATTGGGTTCATCTCT	45.0	45.0	57.2	55.5	810-1041 bp	1.0	58.8