Hotspot

trnF-GAA-ndhJ

LSC · 49146-49733 · 588 bp

Back to Echinochloa

Pi 0.00211

MarkerSeek score 54.80

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 588
variable_sites: 3
pi: 0.00211
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.1	57.9	678-698 bp	1.0	82.0
trnF-GAA-ndhJ_p2	ATGCACAAGAAAGGGTCAGG	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.1	57.9	731-751 bp	1.0	81.5
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	ATAGGCTGGCCCTTACGTAA	50.0	50.0	58.1	58.2	668-688 bp	1.0	81.5
trnF-GAA-ndhJ_p4	ATGCACAAGAAAGGGTCAGG	ATAGGCTGGCCCTTACGTAA	50.0	50.0	58.1	58.2	721-741 bp	1.0	81.0
trnF-GAA-ndhJ_p5	GCACAAGAAAGGGTCAGGAT	TGAAAGTTGGATAGGCTGGC	50.0	50.0	57.8	57.9	729-749 bp	1.0	80.5