Hotspot

atpH-atpF

LSC · 33761-34234 · 474 bp

Back to Echinochloa

Pi 0.00342

MarkerSeek score 48.10

Primer available yes

Label atpH-atpF

Feature Metadata

label_name: atpH-atpF
feature_type: igs
region: LSC
length_bp: 474
variable_sites: 5
pi: 0.00342
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpF_p1	TTATGGACTGGTTGTGGCAC	CGAATGGCCAGTGAGCTAAA	50.0	50.0	58.1	58.3	575 bp	1.0	77.8
atpH-atpF_p2	TGTGGCACTAGCGCTTTTAT	CGAATGGCCAGTGAGCTAAA	45.0	50.0	57.9	58.3	563 bp	1.0	77.7
atpH-atpF_p3	TTGTGGCACTAGCGCTTTTA	CGAATGGCCAGTGAGCTAAA	45.0	50.0	58.1	58.3	564 bp	1.0	77.7
atpH-atpF_p4	TTATGGACTGGTTGTGGCAC	GCCAGTGAGCTAAAAACACG	50.0	50.0	58.1	57.7	569 bp	1.0	77.4
atpH-atpF_p5	TGTGGCACTAGCGCTTTTAT	GCCAGTGAGCTAAAAACACG	45.0	50.0	57.9	57.7	557 bp	1.0	77.3