Hotspot

trnC-GCA-rpoB

LSC · 19471-20615 · 1145 bp

Back to Echinochloa

Pi 0.00374

MarkerSeek score 48.40

Primer available yes

Label trnC-GCA-rpoB

Feature Metadata

label_name: trnC-GCA-rpoB
feature_type: igs
region: LSC
length_bp: 1145
variable_sites: 11
pi: 0.00374
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-rpoB_p1	AAAGGATTTGCAGTCCCCTG	CCGGGTATTGTGGACATTCC	50.0	55.0	58.1	58.3	1229-1235 bp	1.0	77.6
trnC-GCA-rpoB_p2	AAAGGATTTGCAGTCCCCTG	CGGGTATTGTGGACATTCCC	50.0	55.0	58.1	58.3	1228-1234 bp	1.0	77.6
trnC-GCA-rpoB_p3	AAAGGATTTGCAGTCCCCTG	AAGTTCTTCTGCCAAGCCTT	50.0	45.0	58.1	57.6	1293-1299 bp	1.0	77.1
trnC-GCA-rpoB_p4	AAAGGATTTGCAGTCCCCTG	AGTTCTTCTGCCAAGCCTTG	50.0	50.0	58.1	58.4	1292-1298 bp	1.0	77.0
trnC-GCA-rpoB_p5	AAAGGATTTGCAGTCCCCTG	CAAGTTCTTCTGCCAAGCCT	50.0	50.0	58.1	58.4	1294-1300 bp	1.0	77.0