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psbM-petN

LSC · 17561-18378 · 818 bp

Back to Echinochloa

Pi 0.00629

MarkerSeek score 63.50

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: LSC
length_bp: 818
variable_sites: 13
pi: 0.00629
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	TGCTACCGCATTGTTCATTC	TAGTAAGTCTCGCTTGGGCT	45.0	50.0	56.8	58.2	958-980 bp	1.0	73.3
psbM-petN_p2	AAAACAGCCAGCCAAAATGA	TAGTAAGTCTCGCTTGGGCT	40.0	50.0	56.7	58.2	903-925 bp	1.0	72.8
psbM-petN_p3	TGCTACCGCATTGTTCATTCT	TAGTAAGTCTCGCTTGGGCT	42.9	50.0	58.3	58.2	958-980 bp	1.0	72.6
psbM-petN_p4	TGCTACCGCATTGTTCATTC	GTAGTATGGGGGAGGAGTGG	45.0	60.0	56.8	58.3	902-924 bp	1.0	72.5
psbM-petN_p5	TGCTACCGCATTGTTCATTCT	GTAGTATGGGGGAGGAGTGG	42.9	60.0	58.3	58.3	902-924 bp	1.0	71.8