Hotspot

trnD-GUC-psbM

LSC · 16384-17455 · 1072 bp

Back to Echinochloa

Pi 0.00524

MarkerSeek score 45.20

Primer available yes

Label trnD-GUC-psbM

Feature Metadata

label_name: trnD-GUC-psbM
feature_type: igs
region: LSC
length_bp: 1072
variable_sites: 19
pi: 0.00524
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-psbM_p1	TTCAATTGGTCAGAGCACCG	TCATTTTGGCTGGCTGTTTT	50.0	40.0	58.5	56.7	1237-1265 bp	1.0	67.1
trnD-GUC-psbM_p2	TAGGTATGCCATACACCCCG	TCATTTTGGCTGGCTGTTTT	55.0	40.0	58.7	56.7	1269-1297 bp	1.0	65.7
trnD-GUC-psbM_p3	TTCAATTGGTCAGAGCACCG	AGAATGAACAATGCGGTAGC	50.0	45.0	58.5	56.5	1183-1211 bp	1.0	65.6
trnD-GUC-psbM_p4	TTCAATTGGTCAGAGCACCG	AATCATTTTGGCTGGCTGTT	50.0	40.0	58.5	56.5	1239-1267 bp	1.0	65.3
trnD-GUC-psbM_p5	TTCAATTGGTCAGAGCACCG	ATCATTTTGGCTGGCTGTTT	50.0	40.0	58.5	56.5	1238-1266 bp	1.0	65.3